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Slamf7 ELISA Kit (Mouse)

ArtNr OKBB01381
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Konjugat/Tag Biotin
Sensitivity <10 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SLAM family member 7; Leukocyte cell-surface antigen; Novel Ly9; CD319; Slamf7
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
SLAMF7
Gene Fullname
SLAM family member 7
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
SLAM family member 7 is a protein that in humans is encoded by the SLAMF7 gene. This gene is mapped to 1; 1 H3. The surface antigen CD319 (SLAMF7) is a robust marker of normal plasma cells and malignant plasma cells in multiple myeloma. In contrast to CD138 (the traditional plasma cell marker), CD319/SLAMF7 is much more stable and allows robust isolation of malignant plasma cells from delayed or even cryopreserved samples.
Nucleotide accession_num
NM_144539.5
Protein accession_num
NP_653122.2
Protein name
SLAM family member 7
Immunogen
Expression system for standard: NS0; Immunogen sequence: S23-G224
Manufacturer - Specificity
Natural and recombinant mouse SLAMF7
Kit duration
~ 3 Hours
Kit principle
Aviva's mouse SLAMF7 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SLAMF7 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: S23-G224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SLAMF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse SLAMF7 amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum and plasma (heparin, EDTA, citrate)

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Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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