Mdk ELISA Kit (Rat)

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MDK

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

NM_030859.2

Midkine

Natural and recombinant rat Midkine

Aviva's rat Midkine ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Midkine has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: V21-D140) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Midkine is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Midkine amount of sample captured in plate.