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Chrd ELISA Kit (Rat)

ArtNr OKBB01453
Hersteller AVIVA Systems Biology
Menge 96 wells/kit, with removable strips.
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Konjugat/Tag Biotin
Sensitivity <50 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Chordin; Chrd
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
CHRD
Gene Fullname
chordin
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. In humans, the chordin peptide is encoded by the CHRD gene. It is mapped to 11q23. It dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs.
Protein name
Chordin
Immunogen
Expression system for standard: NS0; Immunogen sequence: T27-S948
Manufacturer - Specificity
Natural and recombinant rat Chordin
Kit duration
~ 3 Hours
Kit principle
Aviva's rat Chordin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chordin has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: T27-S948) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chordin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat Chordin amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum and plasma (heparin, EDTA)

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
lieferbar

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