Cd99 ELISA Kit (Mouse)

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CD99

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

NM_025584.2

CD99 antigen

Natural and recombinant mouse CD99

Aviva's mouse CD99 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD99 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: D29-G137) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD99 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD99 amount of sample captured in plate.