APOB ELISA Kit (Mouse)

ELISA Kit

Wet Ice

APOB

4505

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100. Unlike the apoB-48 and apoB-100 structural equivalents in human, which are synthesized exclusively in the gut and liver, respectively, the mouse apoB-48 isoform is also found in mouse liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2179 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination.

NP_033823

This assay has high sensitivity and excellent specificity for detection of
mouse APOB. No significant cross-reactivity or interference between mouse APOB and analogues was observed.

2.5 Hours

Aviva Systems Biology APOB ELISA Kit (Mouse) (OKCA00135) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Mouse APOB and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to APOB and incubated. APOB immobilized in the wells competes with the liquid phase APOB contained in the samples or standards for binding with the HRP-Conjugated APOB Antibody. Wells are washed removing unbound APOB, unbound HRP-Conjugated APOB Antibody or APOB/HRP-Conjugated APOB Antibody complex. HRP-Conjugated APOB Antibody bound to APOB immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated APOB Antibody captured in the well and inversely proportional to the amount of APOB which was contained in the sample or standard.

Intra-assay: CV% < 8% (n = 20). Inter-assay: CV% < 10% (n = 20)