Insulin ELISA Kit (Goat)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

6 kDa

51

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5 region and with the IGF2 gene at the 3 region.

This assay has high sensitivity and excellent specificity for detection of goat INS. No significant cross-reactivity or interference between goat INS and analogues was observed

2.5 Hours

Aviva Systems Biology Insulin ELISA Kit (Goat) (OKCA00271) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A Goat monoclonal antibody specific for Goat Insulin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells and incubated. After washing, a biotinylated detector antibody specific for Goat Insulin is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Goat Insulin captured in well.