NPPB ELISA Kit (Dog)

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NPPB

Store as indicated in product manual.

NM_001009244.1

Natriuretic peptides B

~ 3.5 Hours

Aviva Systems Biology NPPB ELISA Kit (Dog) (OKCA00583) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPPB has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPPB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPPB captured in the well.

Intra-assay Precision: 3 samples with known low, middle and high level GAST were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level GAST were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <15%
Inter-Assay CV: <15%