PAWR ELISA Kit (Human)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

PAWR

Store as indicated in product manual.

NM_002583.2

PRKC apoptosis WT1 regulator protein

~ 3.5 Hours

Aviva Systems Biology PAWR ELISA Kit (Human) (OKCA00758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PAWR has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PAWR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PAWR captured in the well.

Intra-assay Precision: 3 samples with known low, middle and high level PAWR were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level PAWR were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%