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RSPO3 ELISA Kit (Mouse)

RSPO3 ELISA Kit (Mouse)

ArtNr	OKCA00922
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism By Cell Type Obesity ELISA Lipoprotein Receptors & Associated Proteins ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	1.17 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Cri,Thsd,Thsd2,AW742308,Cristin1,2810459H04Rik
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	RSPO3
Gene Fullname	R-spondin 3
Reconstitution and storage	Store as indicated in product manual.
Description of target	Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells.
Nucleotide accession_num	NM_028351.3
Protein accession_num	NP_082627.3
Protein name	R-spondin-3
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit duration	~ 3.5 Hours
Kit linearity	Dilution - Mean n=4 (%) - Range (%) Neat - 90 - 86-94 1:2 - 98 - 93-103 1:4 - 102 - 98-105 1:8 - 94 - 92-96
Kit principle	Aviva Systems Biology Rspo3 ELISA Kit (Mouse) (OKCA00922) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Rspo3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Rspo3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rspo3 captured in well.
Kit recovery	Matrix - Recovery range (%) - Average(%) Serum (n=5) - 97 - 94-100 EDTA Plasma (n=4) - 93 - 90-97
Kit reproducibility	Intra-assay Precision: 3 samples with known low, middle and high level Rspo3 were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level Rspo3 were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10%
Sample Type	Serum, Plasma, Tissue Homogenates

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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OKCA00922-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 30.10.2025

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