APP ELISA Kit (Rat)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

APP

Store as indicated in product manual.

NM_001195669.1

Amyloid beta A4 protein

~ 3.5 Hours

Aviva Systems Biology APP ELISA Kit (Rat) (OKCA00945) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for APP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for APP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample APP captured in well.

Intra-assay Precision: 3 samples with known low, middle and high level APP were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level APP were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%