PODXL ELISA Kit (Rat)

ELISA Kit

Wet Ice

Podocalyxin

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

NP_001076235.1

Assay Methodology: Quantitative Sandwich ELISA

Dilution - Mean n=4 (%) - Range (%)
Neat - 87 - 81-93
1:2 - 97 - 93-101
1:4 - 89 - 85-93
1:8 - 98 - 92-104

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 91 - 85-97
EDTA Plasma (n=4) - 89 - 83-94

Serum, Urine, Cell Culture Supernatant