NOS2 ELISA Kit (Rat)

ArtNr	OKCA02234
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Molecular Targets for Neuroscience Aging & Neurological Disorders Protein Aggregation, Clearance & Misfolding Neural Development By Cell Type Carcinoma Hematology Leukemia Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma Cytoskeleton ELISA ELISA & Immunoassays ELISA Kits Immunoassay Intermediate filaments Microglia Markers Stem Cell Markers Oligodendrocyte Markers
Typ	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.195 IU/mL.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	NOS2A
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	NOS2
Reconstitution and storage	Store as indicated in product manual.
Description of target	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
Protein name	Nitric oxide synthase, inducible
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit duration	~ 3.5 Hours
Kit linearity	Sample Type - Dilution - Mean (%) - Range (%) Serum (n=4) - Neat - 96 - 90-100 Serum (n=4) - 1:2 - 94 - 89-99 Serum (n=4) - 1:4 - 100 - 95-104 Serum (n=4) - 1:8 - 102 - 98-105
Kit principle	Aviva Systems Biology NOS2 ELISA Kit (Rat) (OKCA02234) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NOS2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NOS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NOS2 captured in well.
Kit recovery	Sample Type - Mean (%) - Range (%) Serum (n=5) - 91 - 89-95 EDTA plasma (n=4) - 93 - 89-97
Kit reproducibility	Mean Intra-Assay Variation <10% CV (n=20) Mean Inter-Assay Variation <12% CV (n=8)
Sample Type	Serum, Plasma And Tissue Homogenates

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OKCA02234.pdf

OKCA02234-datasheet.pdf

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Menge: 96 Wells

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