P4HB ELISA Kit (Mouse)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

P4HB

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.

NP_035162

Assay Methodology: Quantitative Sandwich Immunoassay

Dilution - Mean Recovery (%) - Range (%)
Neat - 94 - 87-98
1:2 - 103 - 96-108
1:4 - 92 - 85-97
1:8 - 101 - 95-107

Sample Type - Mean Recovery (%) - Range (%)
Serum (n=5) - 89 - 84-95
EDTA Plasma (n=4) - 99 - 91-105

Serum, Plasma, Tissue Homogenates