HTRA1 ELISA Kit (Mouse)

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HTRA1

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves or not. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

NP_062510

Assay Methodology: Quantitative Sandwich Immunoassay

Dilution - Mean Recovery (%) - Range (%)
Neat - 89 - 85-93
1:2 - 95 - 90-100
1:4 - 91 - 86-95
1:8 - 97 - 92-101

Sample Type - Mean Recovery (%) - Range (%)
Serum (n=5) - 104 - 100-110
EDTA Plasma (n=4) - 93 - 89-97

Serum, Plasma, Tissue Homogenates