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MPST ELISA Kit (Mouse)

MPST ELISA Kit (Mouse)

ArtNr	OKCD00343
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Cell Biology Cell Status Cell Metabolism ELISA Oxidative Stress ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	< 0.054 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	3-mercaptopyruvate sulfurtransferase;Mst.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Mpst
Gene Fullname	mercaptopyruvate sulfurtransferase
Protein size	297
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Detoxifies cyanide and is required for thiosulfate biosynthesis. Acts as an antioxidant. In combination with cysteine aminotransferase (CAT), contributes to the catabolism of cysteine and is an important producer of hydrogen sulfide in the brain, retina and vascular endothelial cells. Hydrogen sulfide H(2)S is an important synaptic modulator, signaling molecule, smooth muscle contractor and neuroprotectant. Its production by the 3MST/CAT pathway is regulated by calcium ions.
Nucleotide accession_num	NM_001162492.1
Protein accession_num	NP_001155964
Protein name	3-mercaptopyruvate sulfurtransferase
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Mercaptopyruvate Sulfurtransferase (MPST).No significant cross-reactivity or interference between Mercaptopyruvate Sulfurtransferase (MPST) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components	Component: Amount Anti-Mpst Microplate: 96 Wells (12 x 8 Well Strips) Mpst Lyophilized Standard: 2 x 10 ng 100X Biotinylated Mpst Detector Antibody: 120 uL Avidin/HRP Conjugate: 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Mercaptopyruvate Sulfurtransferase (MST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Mercaptopyruvate Sulfurtransferase (MST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Mercaptopyruvate Sulfurtransferase (MST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 3-Mercaptopyruvate Sulfurtransferase (MST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-Mercaptopyruvate Sulfurtransferase (MST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-Mercaptopyruvate Sulfurtransferase (MST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

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OKCD00343.pdf

OKCD00343-datasheet.pdf

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Menge: 96 Wells

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