HIPK2 ELISA Kit (Human) (OKCD00468)

homeodomain-interacting protein kinase 2

28996

Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A. Mediates transcriptional activation of TP73. In response to TGFB, cooperates with DAXX to activate JNK. Negative regulator through phosphorylation and subsequent proteasomal degradation of CTNNB1 and the antiapoptotic factor CTBP1. In the Wnt/beta-catenin signaling pathway acts as an intermediate kinase between MAP3K7/TAK1 and NLK to promote the proteasomal degradation of MYB. Phosphorylates CBX4 upon DNA damage and promotes its E3 SUMO-protein ligase activity. Activates CREB1 and ATF1 transcription factors by phosphorylation in response to genotoxic stress. In response to DNA damage, stabilizes PML by phosphorylation. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation. Promotes angiogenesis, and is involved in erythroid differentiation, especially during fetal liver erythropoiesis. Phosphorylation of RUNX1 and EP300 stimulates EP300 transcription regulation activity. Triggers ZBTB4 protein degradation in response to DNA damage. Modulates HMGA1 DNA-binding affinity. In response to high glucose, triggers phosphorylation-mediated subnuclear localization shifting of PDX1. Involved in the regulation of eye size, lens formation and retinal lamination during late embryogenesis.18 Publications < p>Manually curated information for which there is published experimental evidence.< /p> < p>< a href="/manual/evidences#ECO:0000269">Moreâa‚¬A< /a>< /p> Manual assertion based on experiment iniRef.8"Identification and characterization of HIPK2 interacting with p73 and modulating functions of the p53 family in vivo."_x005F_x000D_ Kim E.-J., Park J.-S., Um S.-J._x005F_x000D_ J. Biol. Chem. 277:32020-32028(2002) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH TP73; TP53 AND TP63, MUTAGENESIS OF LYS-228, FUNCTION.Ref.9"Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2."_x005F_x000D_ Hofmann T.G., Moeller A., Sirma H., Zentgraf H., Taya Y., Droege W., Will H., Schmitz M.L._x005F_x000D_ Nat. Cell Biol. 4:1-10(2002) [PubMed] [Europe PMC] [Abstract]Cited for: SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, INTERACTION WITH TP53 AND CREBBP, MUTAGENESIS OF LYS-228, FUNCTION, INDUCTION.Ref.11"HIPK2 regulates transforming growth factor-beta-induced c-Jun NH(2)-terminal kinase activation and apoptosis in human hepatoma cells."_x005F_x000D_ Hofmann T.G., Stollberg N., Schmitz M.L., Will H._x005F_x000D_ Cancer Res. 63:8271-8277(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH DAXX, MUTAGENESIS OF LYS-228.Ref.12"TP53INP1s and homeodomain-interacting protein kinase-2 (HIPK2) are partners in regulating p53 activity."_x005F_x000D_ Tomasini R., Samir A.A., Carrier A., Isnardon D., Cecchinelli B., Soddu S., Malissen B., Dagorn J.-C., Iovanna J.L., Dusetti N.J._x005F_x000D_ J. Biol. Chem. 278:37722-37729(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH P53DINP1.Ref.13"Requirement of the co-repressor homeodomain-interacting protein kinase 2 for ski-mediated inhibition of bone morphogenetic protein-induced transcriptional activation."_x005F_x000D_ Harada J., Kokura K., Kanei-Ishii C., Nomura T., Khan M.M., Kim Y., Ishii S._x005F_x000D_ J. Biol. Chem. 278:38998-39005(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, INTERACTION WITH SKI; SMAD1; SMAD2 AND SMAD3, MUTAGENESIS OF LYS-228 AND 359-SER--TYR-361.Ref.17"Phosphorylation-dependent control of Pc2 SUMO E3 ligase activity by its substrate protein HIPK2."_x005F_x000D_ Roscic A., Moeller A., Calzado M.A., Renner F., Wimmer V.C., Gresko E., Luedi K.S., Schmitz M.L._x005F_x000D_ Mol. Cell 24:77-89(2006) [PubMed] [Europe PMC] [Abstract]Cited for: INTERACTION WITH CBX4, SUMOYLATION AT LYS-32, FUNCTION.Ref.18"Homeodomain-interacting protein kinase-2 (HIPK2) phosphorylates HMGA1a at Ser-35, Thr-52, and Thr-77 and modulates its DNA binding affinity."_x005F_x000D_ Zhang Q., Wang Y._x005F_x000D_ J. Proteome Res. 6:4711-4719(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS HMGA1 KINASE.Ref.19"PEBP2-beta/CBF-beta-dependent phosphorylation of RUNX1 and p300 by HIPK2: implications for leukemogenesis."_x005F_x000D_ Wee H.-J., Voon D.C.-C., Bae S.-C., Ito Y._x005F_x000D_ Blood 112:3777-3787(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS RUNX1 AND EP300 KINASE, MUTAGENESIS OF LYS-228.Ref.21"PML activates transcription by protecting HIPK2 and p300 from SCFFbx3-mediated degradation."_x005F_x000D_ Shima Y., Shima T., Chiba T., Irimura T., Pandolfi P.P., Kitabayashi I._x005F_x000D_ Mol. Cell. Biol. 28:7126-7138(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, UBIQUITINATION BY FBXO3.Ref.23"Transcriptional regulation of hypoxia-inducible factor 1alpha by HIPK2 suggests a novel mechanism to restrain tumor growth."_x005F_x000D_ Nardinocchi L., Puca R., Guidolin D., Belloni A.S., Bossi G., Michiels C., Sacchi A., Onisto M., D'Orazi G._x005F_x000D_ Biochim. Biophys. Acta 1793:368-377(2009) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS HIF1A TRANSCRIPTION REGULATOR AND ANGIOGENESIS PROMOTER.Ref.25"PML tumor suppressor is regulated by HIPK2-mediated phosphorylation in response to DNA damage."_x005F_x000D_ Gresko E., Ritterhoff S., Sevilla-Perez J., Roscic A., Froebius K., Kotevic I., Vichalkovski A., Hess D., Hemmings B.A., Schmitz M.L._x005F_x000D_ Oncogene 28:698-708(2009) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS PML KINASE, INTERACTION WITH PML, MUTAGENESIS OF LYS-228.Ref.26"The human protein kinase HIPK2 phosphorylates and downregulates the methyl-binding transcription factor ZBTB4."_x005F_x000D_ Yamada D., Perez-Torrado R., Filion G., Caly M., Jammart B., Devignot V., Sasai N., Ravassard P., Mallet J., Sastre-Garau X., Schmitz M.L., Defossez P.A._x005F_x000D_ Oncogene 28:2535-2544(2009) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS ZBTB4 KINASE, INTERACTION WITH ZBTB4.Ref.28"Pancreatic and duodenal homeobox 1 (PDX1) phosphorylation at serine-269 is HIPK2-dependent and affects PDX1 subnuclear localization."_x005F_x000D_ An R., da Silva Xavier G., Semplici F., Vakhshouri S., Hao H.X., Rutter J., Pagano M.A., Meggio F., Pinna L.A., Rutter G.A._x005F_x000D_ Biochem. Biophys. Res. Commun. 399:155-161(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS PDX1 KINASE.Ref.29"Homeodomain-interacting protein kinase 2 (HIPK2) targets beta-catenin for phosphorylation and proteasomal degradation."_x005F_x000D_ Kim E.-A., Kim J.E., Sung K.S., Choi D.W., Lee B.J., Choi C.Y._x005F_x000D_ Biochem. Biophys. Res. Commun. 394:966-971(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS CTNNB1 KINASE.Ref.30"Transcriptional regulation of ferritin and antioxidant genes by HIPK2 under genotoxic stress."_x005F_x000D_ Hailemariam K., Iwasaki K., Huang B.W., Sakamoto K., Tsuji Y._x005F_x000D_ J. Cell Sci. 123:3863-3871(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS ATF1 KINASE, INTERACTION WITH ATF1.Ref.31"Regulation of genotoxic stress response by homeodomain-interacting protein kinase 2 through phosphorylation of cyclic AMP response element-binding protein at serine 271."_x005F_x000D_ Sakamoto K., Huang B.-W., Iwasaki K., Hailemariam K., Ninomiya-Tsuji J., Tsuji Y._x005F_x000D_ Mol. Biol. Cell 21:2966-2974(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION AS CREB1 KINASE, INTERACTION WITH CREB1.Ref.33"Role of the SUMO-interacting motif in HIPK2 targeting to the PML nuclear bodies and regulation of p53."_x005F_x000D_ Sung K.S., Lee Y.A., Kim E.T., Lee S.R., Ahn J.H., Choi C.Y._x005F_x000D_ Exp. Cell Res. 317:1060-1070(2011) [PubMed] [Europe PMC] [Abstract]Cited for: SUBCELLULAR LOCATION, SUMOYLATION, FUNCTION, MUTAGENESIS OF 885-VAL--ILE-888 AND 892-SER--ASP-895.Ref.38"DNA damage-induced heterogeneous nuclear ribonucleoprotein K SUMOylation regulates p53 transcriptional activation."_x005F_x000D_ Pelisch F., Pozzi B., Risso G., Munoz M.J., Srebrow A._x005F_x000D_ J. Biol. Chem. 287:30789-30799(2012) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION.

< 0.061 ng/mL