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CHRDL1 ELISA Kit (Mouse)

ArtNr OKCD00473
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity < 0.054 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CHL;CHL1;chordin-like protein 1;N;neuralin-1;neurogenesin-1;Nrln1;V;Ventroptin;ventroptin-alpha;ventroptin-beta;VOPT.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Chrdl1
Gene Fullname
chordin-like 1
Protein size
447
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation (By similarity). Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development (By similarity).
Nucleotide accession_num
NM_001114385.1
Protein accession_num
NP_001107857
Protein name
Chordin-like protein 1
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of Chordin Like Protein 1 (CHRDL1).No significant cross-reactivity or interference between Chordin Like Protein 1 (CHRDL1) and analogues was observed.
Assay info
Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components
Component: Amount
Anti-Chrdl1 Microplate: 96 Wells (12 x 8 Well Strips)
Chrdl1 Lyophilized Standard: 2 x 10 ng
100X Biotinylated Chrdl1 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
- 87-94% - 83-90% - 92-105% - 92-105%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chordin Like Protein 1 (CHRDL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chordin Like Protein 1 (CHRDL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chordin Like Protein 1 (CHRDL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chordin Like Protein 1 (CHRDL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery Range (%) - Average (%)
- 80-93 - 80
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chordin Like Protein 1 (CHRDL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chordin Like Protein 1 (CHRDL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates, cell lysates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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