TNS3 ELISA Kit (Human)

ELISA Kit

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TNS3

1445

May play a role in actin remodeling. Involved in the dissociation of the integrin-tensin-actin complex. EGF activates TNS4 and down-regulates TNS3 which results in capping the tail of ITGB1. Seems to be involved in mammary cell migration. May be involved in cell migration and bone development (By similarity).

NP_073585

This assay has high sensitivity and excellent specificity for detection of Tensin 3 (TNS3).No significant cross-reactivity or interference between Tensin 3 (TNS3) and analogues was observed.

Component: Amount
Anti-TNS3 Microplate: 96 Wells (12 x 8 Well Strips)
TNS3 Lyophilized Standard: 2 x 10 ng
100X Biotinylated TNS3 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tensin 3 (TNS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tensin 3 (TNS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tensin 3 (TNS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tensin 3 (TNS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tensin 3 (TNS3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tensin 3 (TNS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%