KIAA1524 ELISA Kit (Human)

ELISA Kit

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CIP2A

905

Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.

NP_065941

This assay has high sensitivity and excellent specificity for detection of Cancerous Inhibitor Of PP2A (CIP2A).No significant cross-reactivity or interference between Cancerous Inhibitor Of PP2A (CIP2A) and analogues was observed.

Component: Amount
Anti-KIAA1524 Microplate: 96 Wells (12 x 8 Well Strips)
KIAA1524 Lyophilized Standard: 2 x 10 ng
100X Biotinylated KIAA1524 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cancerous Inhibitor Of PP2A (CIP2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cancerous Inhibitor Of PP2A (CIP2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cancerous Inhibitor Of PP2A (CIP2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cancerous Inhibitor Of PP2A (CIP2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cancerous Inhibitor Of PP2A (CIP2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cancerous Inhibitor Of PP2A (CIP2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%