IRAK4 ELISA Kit (Human) (OKCD00674)

interleukin-1 receptor-associated kinase 4

51135

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.9 Publications < p>Manually curated information for which there is published experimental evidence.< /p> < p>< a href="/manual/evidences#ECO:0000269">Moreâa‚¬A< /a>< /p> Manual assertion based on experiment iniRef.1"IRAK4: a novel member of the IRAK family with the properties of an IRAK-kinase."_x005F_x000D_ Li S., Strelow A., Fontana E.J., Wesche H._x005F_x000D_ Proc. Natl. Acad. Sci. U.S.A. 99:5567-5572(2002) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, INTERACTION WITH IRAK1 AND TRAF6.Ref.9"Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4."_x005F_x000D_ Burns K., Janssens S., Brissoni B., Olivos N., Beyaert R., Tschopp J._x005F_x000D_ J. Exp. Med. 197:263-268(2003) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF IRAK1.Ref.11"IRAK4 kinase activity is redundant for interleukin-1 (IL-1) receptor-associated kinase phosphorylation and IL-1 responsiveness."_x005F_x000D_ Qin J., Jiang Z., Qian Y., Casanova J.-L., Li X._x005F_x000D_ J. Biol. Chem. 279:26748-26753(2004) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION, MUTAGENESIS OF LYS-213.Ref.15"Cross-talk between IRAK-4 and the NADPH oxidase."_x005F_x000D_ Pacquelet S., Johnson J.L., Ellis B.A., Brzezinska A.A., Lane W.S., Munafo D.B., Catz S.D._x005F_x000D_ Biochem. J. 403:451-461(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF NCF1.Ref.16"IRAK-4 kinase activity is required for interleukin-1 (IL-1) receptor- and toll-like receptor 7-mediated signaling and gene expression."_x005F_x000D_ Koziczak-Holbro M., Joyce C., Gluck A., Kinzel B., Muller M., Tschopp C., Mathison J.C., Davis C.N., Gram H._x005F_x000D_ J. Biol. Chem. 282:13552-13560(2007) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN TLR7 SIGNALING PATHWAY.Ref.18"The IRAK-catalysed activation of the E3 ligase function of Pellino isoforms induces the Lys63-linked polyubiquitination of IRAK1."_x005F_x000D_ Ordureau A., Smith H., Windheim M., Peggie M., Carrick E., Morrice N., Cohen P._x005F_x000D_ Biochem. J. 409:43-52(2008) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF PELI1.Ref.23"IRAK1 and IRAK4 promote phosphorylation, ubiquitination, and degradation of MyD88 adaptor-like (Mal)."_x005F_x000D_ Dunne A., Carpenter S., Brikos C., Gray P., Strelow A., Wesche H., Morrice N., O'Neill L.A._x005F_x000D_ J. Biol. Chem. 285:18276-18282(2010) [PubMed] [Europe PMC] [Abstract]Cited for: FUNCTION IN PHOSPHORYLATION OF TIRAP.Ref.34"TLR9 activation induces normal neutrophil responses in a child with IRAK-4 deficiency: involvement of the direct PI3K pathway."_x005F_x000D_ Hoarau C., Gerard B., Lescanne E., Henry D., Francois S., Lacapere J.J., El Benna J., Dang P.M., Grandchamp B., Lebranchu Y., Gougerot-Pocidalo M.A., Elbim C._x005F_x000D_ J. Immunol. 179:4754-4765(2007) [PubMed] [Europe PMC] [Abstract]Cited for: VARIANT IRAK4D CYS-12, CHARACTERIZATION OF VARIANT IRAK4D CYS-12, VARIANT HIS-391, FUNCTION.Ref.38"Functional assessment of the mutational effects of human IRAK4 and MyD88 genes."_x005F_x000D_ Yamamoto T., Tsutsumi N., Tochio H., Ohnishi H., Kubota K., Kato Z., Shirakawa M., Kondo N._x005F_x000D_ Mol. Immunol. 58:66-76(2014) [PubMed] [Europe PMC] [Abstract]Cited for: CHARACTERIZATION OF VARIANTS VAL-5; TRP-20; THR-26; VAL-39 AND ARG-98, CHARACTERIZATION OF VARIANTS IRAK4D CYS-12 AND ASP-298, FUNCTION, INTERACTION WITH MYD88.

< 0.64 ng/mL