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MKI67 ELISA Kit (Mouse)

MKI67 ELISA Kit (Mouse)

ArtNr	OKCD00857
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Lineage Markers Molecular Targets for Neuroscience Neural Development By Cell Type Blastoma Carcinoma Hematology Lymphoma Cell Biology Cell Status ELISA Proliferation Viability ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Bladder Cancer Breast Cancer Testicular & Prostate Cancer Immunostaining Cytoskeleton Markers
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	< 0.119 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	antigen identified by monoclonal antibody Ki-67 homolog;antigen Ki67 homolog;antigen KI-67 homolog;D630048A14Rik;Ki6;Ki-6;Ki67;Ki-67;proliferation marker protein Ki-67.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Mki67
Gene Fullname	antigen identified by monoclonal antibody Ki 67
Protein size	3177
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (By similarity). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
Nucleotide accession_num	NM_001081117.2
Protein accession_num	NP_001074586
Protein name	Proliferation marker protein Ki-67
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Ki-67 Protein (Ki67P).No significant cross-reactivity or interference between Ki-67 Protein (Ki67P) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components	Component: Amount Anti-Mki67 Microplate: 96 Wells (12 x 8 Well Strips) Mki67 Lyophilized Standard: 2 x 20 ng 100X Biotinylated Mki67 Detector Antibody: 120 uL Avidin/HRP Conjugate: 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ki-67 Protein (Ki-67). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ki-67 Protein (Ki-67). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ki-67 Protein (Ki-67), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Ki-67 Protein (Ki-67) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ki-67 Protein (Ki-67) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD00857.pdf

OKCD00857-datasheet.pdf

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Menge: 96 Wells

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