CLIC4 ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

chloride intracellular channel 4

2°C to 8°C|-20°C

NM_013943.2

Chloride intracellular channel protein 4

Assay Methodology: Quantitative Sandwich Immunoassay

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chloride Intracellular Channel Protein 4 (CLIC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chloride Intracellular Channel Protein 4 (CLIC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates, cell lysates and other biological fluids.