TRPV3 ELISA Kit (Human)

ELISA Kit

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TRPV3

790

Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen).

NP_659505

This assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3).No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3) and analogues was observed.

Component: Amount
Anti-TRPV3 Microplate: 96 Wells (12 x 8 Well Strips)
TRPV3 Lyophilized Standard: 2 x 50 ng
100X Biotinylated TRPV3 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%