Pcsk1 ELISA Kit (Rat)

ELISA Kit

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Pcsk1

752

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.

NP_058787.1

This assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1).No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) and analogues was observed.

Component: Amount
Anti-Pcsk1 Microplate: 96 Wells (12 x 8 Well Strips)
Pcsk1 Lyophilized Standard: 2 x 200 ng
100X Biotinylated Pcsk1 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%