EFEMP2 ELISA Kit (Human)

ELISA Kit

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EFEMP2

443

EGF-containing fibulin-like extracellular matrix protein 2

NP_058634.4

This assay has high sensitivity and excellent specificity for detection of Fibulin 4 (FBLN4).No significant cross-reactivity or interference between Fibulin 4 (FBLN4) and analogues was observed.

Component: Amount
Anti-EFEMP2 Microplate: 96 Wells (12 x 8 Well Strips)
EFEMP2 Lyophilized Standard: 2 x 200 ng
100X Biotinylated EFEMP2 Detector Antibody: 120 uL
Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 4 (FBLN4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 4 (FBLN4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 4 (FBLN4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibulin 4 (FBLN4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 4 (FBLN4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 4 (FBLN4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%