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NOD2 ELISA Kit (Human)

NOD2 ELISA Kit (Human)

ArtNr	OKCD02031
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Cholesterol & Lipid Metabolism Targets for Rodent Neurosciences Neural Signaling By Cell Type Carcinoma Hematology Leukemia Lymphoma Myeloma Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines Cell Status Apoptosis ELISA Autophagy ELISA & Immunoassays ELISA Kits Immunoassay By Organ Breast Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.055 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ACUG;BLAU;BLAUS;CARD15;caspase recruitment domain family,member 15;caspase recruitment domain protein 15;caspase recruitment domain-containing protein 15;CD;CLR16.3;IBD1;inflammatory bowel disease protein 1;NLR family,CARD domain containing 2;NLRC2;NOD2B;NOD-like receptor C2;nucleotide-binding oligomerization domain 2;nucleotide-binding oligomerization domain,leucine rich repeat and CARD domain containing 2;nucleotide-binding oligomerization domain-containing protein 2;PSORAS1;YAOS.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	NOD2
Gene Fullname	nucleotide binding oligomerization domain containing 2
Protein size	1040
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561). Component of an autophagy-mediated antibacterial pathway together with ATG16L1 (PubMed:20637199). Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon (PubMed:19701189).
Nucleotide accession_num	NM_001293557.1
Protein accession_num	NP_001280486.1
Protein name	Nucleotide-binding oligomerization domain-containing protein 2
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2).No significant cross-reactivity or interference between Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich Immunoassay
Kit Components	Component: Amount Anti-NOD2 Microplate: 96 Wells (12 x 8 Well Strips) NOD2 Lyophilized Standard: 2 x 10 ng 100X Biotinylated NOD2 Detector Antibody: 120 uL Avidin/HRP Conjugate: 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

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OKCD02031.pdf

OKCD02031-datasheet.pdf

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Menge: 96 Wells

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