Docosahexaenoic Acid ELISA Kit

Enzyme-linked Immunosorbent assay-Competitive

Wet Ice

2°C to 8°C|-20°C

Docosahexaenoic Acid

Component: Amount
Anti-Docosahexaenoic Acid Microplate: 96 Wells (12 x 8 Well Strips)
Docosahexaenoic Acid Lyophilized Standard: 2
100X Docosahexaenoic Acid Biotin Complex: 120 uL
100X Avidin/HRP Conjugate: 120 uL
Standard Diluent: 1 x 20 mL
Biotin Complex Diluent: 1 x 12 mL
Avidin/HRP Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 9 mL
Stop Solution: 1 x 6 mL

2h

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Docosahexaenoic Acid (DHA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Docosahexaenoic Acid (DHA) and unlabeled Docosahexaenoic Acid (DHA) (Standards or samples) with the pre-coated antibody specific to Docosahexaenoic Acid (DHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Docosahexaenoic Acid (DHA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Docosahexaenoic Acid (DHA) in the sample.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Docosahexaenoic Acid (DHA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Docosahexaenoic Acid (DHA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%