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C5 ELISA Kit (Rat)

ArtNr OKCD02318
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity 0.148 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias C5a,Complement C5,Hc,RGD1561905
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
C5
Gene Fullname
complement C5
Protein size
77
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Nucleotide accession_num
XM_008761798.1
Protein accession_num
XP_008760020.1
Protein name
Complement C5
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Component 5 (C5).No significant cross-reactivity or interference between Complement Component 5 (C5) and analogues was observed.
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Anti-C5 Microplate: 96 Wells (12 x 8 Well strips)
C5 Lyophilized Standard: 2
100X Biotinylated C5 Detector Antibody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
Stop Solution: 1 x 6 mL
TMB Substrate: 1 x 9 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 96-104% - 78-94% - 87-103% - 98-105%
EDTA Plasma (n=5) - 79-98% - 86-94% - 88-102% - 79-89%
Heparin Plasma (n=5) - 91-99% - 82-92% - 97-104% - 86-93%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5 (C5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 5 (C5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5 (C5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 5 (C5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 96-104 - 101
EDTA Plasma (n=5) - 90-97 - 94
Heparin Plasma(n=5) - 88-101 - 91
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 5 (C5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 5 (C5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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Lieferung vsl. bis 30.10.2025 

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