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CTSD ELISA Kit (Rat)

ArtNr OKCD02359
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Rat (Rattus norvegicus)
Sensitivity 28.4 pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Cathepsin D
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
CTSD
Gene Fullname
cathepsin D
Protein size
407
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.
Nucleotide accession_num
NM_134334.2
Protein accession_num
NP_599161.2
Protein name
Cathepsin D
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of Cathepsin D (CTSD).No significant cross-reactivity or interference between Cathepsin D (CTSD) and analogues was observed.
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Anti-CTSD Microplate: 96 Wells (12 x 8 Well strips)
CTSD Lyophilized Standard: 2
100X Biotinylated CTSD Detector Antibody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
Stop Solution: 1 x 6 mL
TMB Substrate: 1 x 9 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 87-99% - 83-105% - 83-103% - 78-99%
EDTA Plasma (n=5) - 79-94% - 81-95% - 84-93% - 79-89%
Heparin Plasma (n=5) - 79-95% - 82-94% - 83-96% - 91-101%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin D (CTSD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin D (CTSD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cathepsin D (CTSD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 96-103 - 101
EDTA Plasma (n=5) - 90-97 - 94
Heparin Plasma(n=5) - 80-95 - 90
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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Lieferung vsl. bis 30.10.2025 

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