GHRL ELISA Kit (Pig)

ArtNr	OKCD02571
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Obesity ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Pig (Porcine, Sus scrofa domesticus), Wild Boar
Sensitivity	< 52.9 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	appetite-regulating hormone;GH releasing peptide;ghrelin;GRHL;growth hormone secretagogue;growth hormone-releasing peptide;motilin-related peptide;preproghrelin.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Competitive
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	118 kDa
Gene symbol	GHRL
Gene Fullname	ghrelin and obestatin prepropeptide
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation (By similarity).
Nucleotide accession_num	NM_213807.2
Protein accession_num	NP_998972.1
Protein name	Appetite-regulating hormone
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Ghrelin (GHRL). No significant cross-reactivity or interference between Ghrelin (GHRL) and analogues was observed.
Assay info	Assay Methodology: Quantitative Competitive Inhibition ELISA
Kit Components	Component: Amount Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet Copy From Booklet: Copy From Booklet
Kit detection	Colorimetric
Kit duration	2h
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 Serum (n=5) - 98-105% - 91-99% - 92-101% - 82-95% EDTA Plasma (n=5) - 78-95% - 91-99% - 96-103% - 78-89% Heparin Plasma (n=5) - 99-105% - 92-105% - 80-88% - 91-102%
Kit principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin (GHRL) and unlabeled Ghrelin (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin (GHRL) in the sample.
Kit recovery	Matrix - Recovery range (%) - Average(%) Serum (n=5) - 83-103 - 89 EDTA Plasma (n=5) - 78-104 - 97 Heparin Plasma (n=5) - 86-101 - 95
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrelin (GHRL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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OKCD02571.pdf

OKCD02571-datasheet.pdf

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