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STAT1 ELISA Kit (Human)

STAT1 ELISA Kit (Human)

ArtNr	OKCD02896
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology Neuroscience Neural Lineage Markers Targets for Rodent Neurosciences Neural Signaling Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma Immunostaining Calcium Binding Protein Markers Interneuron Markers
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Sensitivity	0.115 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;signal transducer and activator of transcription 1,91kD;signal transducer and activator of transcription 1,91kDa;signal transducer and activator of transcription 1-alpha/beta;STAT91;transcription factor ISGF-3 components p91/p84.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	STAT1
Gene Fullname	signal transducer and activator of transcription 1
Protein size	750
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Nucleotide accession_num	NM_007315.3
Protein accession_num	NP_009330.1
Protein name	Signal transducer and activator of transcription 1-alpha/beta
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 1 (STAT1).No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 1 (STAT1) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Anti-STAT1 Microplate: 96 Wells (12 x 8 Well strips) STAT1 Lyophilized Standard: 2 100X Biotinylated STAT1 Detector Antibody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL Stop Solution: 1 x 6 mL TMB Substrate: 1 x 9 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 1 (STAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Signal Transducer And Activator Of Transcription 1 (STAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

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OKCD02896.pdf

OKCD02896-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 96 Wells

lieferbar

Lieferung vsl. bis 13.11.2025