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TXNIP ELISA Kit (Mouse)

TXNIP ELISA Kit (Mouse)

ArtNr	OKCD02921
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology By Cell Type Carcinoma Hematology Lymphoma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	0.117 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	1200008J08Rik;AA682105;Hyp;hyperlipidemia 1;Hyplip1;mVDU;Tbp-2;THI;THIF;thioredoxin binding protein-2;thioredoxin-interacting protein;upregulated by 1,25-dihydroxyvitamin D-3;VDU;VDUP1;vitamin D3 up-regulated protein 1.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Txnip
Gene Fullname	thioredoxin interacting protein
Protein size	397
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1) (By similarity). Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth.
Nucleotide accession_num	NM_001009935.2
Protein accession_num	NP_001009935.1
Protein name	Thioredoxin-interacting protein
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of Thioredoxin Binding Protein 2 (TBP2).No significant cross-reactivity or interference between Thioredoxin Binding Protein 2 (TBP2) and analogues was observed.
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Anti-TXNIP Microplate: 96 Wells (12 x 8 Well strips) TXNIP Lyophilized Standard: 2 100X Biotinylated TXNIP Detector Antibody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL Stop Solution: 1 x 6 mL TMB Substrate: 1 x 9 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin-interacting Protein (TXNIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin-interacting Protein (TXNIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin-interacting Protein (TXNIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Thioredoxin-interacting Protein (TXNIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin-interacting Protein (TXNIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin-interacting Protein (TXNIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD02921.pdf

OKCD02921-datasheet.pdf

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Menge: 96 Wells

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