Vergleich

TF ELISA Kit (Pig)

ArtNr OKCD02922
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Pig (Porcine, Sus scrofa domesticus), Wild Boar
Sensitivity 0.054 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias coagulation factor III (thromboplastin,tissue factor);tissue factor.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
F3
Gene Fullname
coagulation factor III, tissue factor
Protein size
397
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Nucleotide accession_num
NM_001244653.1
Protein accession_num
NP_001231582.1
Protein name
Tissue factor
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of Tissue Factor (TF).No significant cross-reactivity or interference between Tissue Factor (TF) and analogues was observed.
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Anti-TF Microplate: 96 Wells (12 x 8 Well strips)
TF Lyophilized Standard: 2
100X Biotinylated TF Detector Antibody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
Stop Solution: 1 x 6 mL
TMB Substrate: 1 x 9 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
sodium citrate plasma(n=5) - 90-101% - 81-105% - 79-92% - 84-93%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tissue Factor (TF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tissue Factor (TF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tissue Factor (TF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tissue Factor (TF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery range (%) - Average(%)
sodium citrate plasma(n=5) - 86-101 - 89
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor (TF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor (TF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Plasma

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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Lieferung vsl. bis 30.10.2025 

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