ADIPOQ Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

adiponectin, C1Q and collagen domain containing

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

NP_033735

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 95-104% - 78-96% - 81-105% - 92-101%
EDTA Plasma (n=5) - 86-101% - 84-99% - 85-95% - 97-104%
Heparin Plasma (n=5) - 92-102% - 78-89% - 92-101% - 97-104%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 80-96 - 92
EDTA Plasma (n=5) - 80-92 - 87
Heparin Plasma (n=5) - 87-95 - 91

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids