APP Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

amyloid beta precursor protein

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.

NP_000475

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 97-105% - 82-93% - 93-101% - 97-105%
EDTA Plasma (n=5) - 98-105% - 81-102% - 97-105% - 92-105%
Heparin Plasma (n=5) - 79-101% - 94-102% - 93-104% - 84-101%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 90-102 - 98
EDTA Plasma (n=5) - 92-101 - 96
Heparin Plasma (n=5) - 79-90 - 83

Serum, plasma, cerebrospinal fluid and other biological fluids