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BLMH Chemi-Luminescent ELISA Kit (Human)

BLMH Chemi-Luminescent ELISA Kit (Human)

ArtNr	OKCD03322
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	Oncology By Cell Type Carcinoma Hematology Lymphoma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.156 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	BH;bleomycin hydrolase;BLM hydrolase;BMH.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	BLMH
Gene Fullname	bleomycin hydrolase
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity.
Nucleotide accession_num	NM_000386
Protein accession_num	NP_000377
Protein name	Bleomycin hydrolase
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount BLMH Microplate: 96 Wells (12 x 8 Well strips) BLMH Lyophilized Standard: 2 100X BLMH Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Bleomycin Hydrolase (BLMH). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bleomycin Hydrolase (BLMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bleomycin Hydrolase (BLMH) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD03322.pdf

OKCD03322-datasheet.pdf

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Menge: 96 Wells

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Lieferung vsl. bis 11.12.2025

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