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CCKAR Chemi-Luminescent ELISA Kit (Rabbit)

ArtNr OKCD03361
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Rabbit (Oryctolagus cuniculus)
Sensitivity 0.312 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CCK1-R;CCK-A receptor;CCK-AR;cholecystokinin receptor type A;cholecystokinin-1 receptor;gastric cholecystokinin A receptor.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
CCKAR
Gene Fullname
cholecystokinin A receptor
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system (By similarity).
Nucleotide accession_num
NM_001082383
Protein accession_num
NP_001075852
Protein name
Cholecystokinin receptor type A
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Cckar Microplate: 96 Wells (12 x 8 Well strips)
Cckar Lyophilized Standard: 2
100X Cckar Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin A Receptor (CCKAR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cholecystokinin A Receptor (CCKAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cholecystokinin A Receptor (CCKAR) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin A Receptor (CCKAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin A Receptor (CCKAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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