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CNTN1 Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD03385
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.156 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias contactin-1;F3;glycoprotein gP135;GP135;MYPCN;neural cell surface protein F3.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
CNTN1
Gene Fullname
contactin 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth (By similarity).
Nucleotide accession_num
NM_001256063
Protein accession_num
NP_001242992
Protein name
Contactin-1
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
CNTN1 Microplate: 96 Wells (12 x 8 Well strips)
CNTN1 Lyophilized Standard: 2
100X CNTN1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Contactin 1 (CNTN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Contactin 1 (CNTN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Contactin 1 (CNTN1) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Contactin 1 (CNTN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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