CX3CL1 Chemi-Luminescent ELISA Kit (Rat)

ELISA Kit

Wet Ice

C-X3-C motif chemokine ligand 1

Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.

NP_604450

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 96-105% - 94-101% - 95-104% - 89-101%
EDTA Plasma (n=5) - 78-103% - 78-102% - 97-104% - 80-97%
Heparin Plasma (n=5) - 83-101% - 78-103% - 80-93% - 88-102%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 95-102 - 98
EDTA Plasma (n=5) - 86-95 - 91
Heparin Plasma (n=5) - 91-99 - 96

Serum, plasma and other biological fluids