EBI3 Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

Epstein-Barr virus induced gene 3

Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines.

NP_056581

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 96-104% - 89-98% - 78-105% - 83-97%
EDTA Plasma (n=5) - 87-104% - 80-95% - 96-103% - 89-97%
Heparin Plasma (n=5) - 86-93% - 99-105% - 93-101% - 82-94%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 83-97 - 88
EDTA Plasma (n=5) - 79-102 - 90
Heparin Plasma (n=5) - 81-92 - 88

Serum, plasma and other biological fluids