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FBLN1 Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD03449
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.469 ng/Ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias FBLN;FIBL1;fibulin-1.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
FBLN1
Gene Fullname
fibulin 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
Nucleotide accession_num
NM_001996
Protein accession_num
NP_001987
Protein name
Fibulin-1
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
FBLN1 Microplate: 96 Wells (12 x 8 Well strips)
FBLN1 Lyophilized Standard: 2
100X FBLN1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 94-102% - 88-96% - 81-104% - 85-96%
EDTA Plasma (n=5) - 90-104% - 96-103% - 79-105% - 78-103%
Heparin Plasma (n=5) - 91-101% - 95-105% - 95-102% - 96-104%
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Fibulin 1 (FBLN1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Fibulin 1 (FBLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Fibulin 1 (FBLN1) level in the sample or standard.
Kit recovery
Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 86-95 - 91
EDTA Plasma (n=5) - 92-104 - 99
Heparin Plasma (n=5) - 78-97 - 84
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 1 (FBLN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 1 (FBLN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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