HIF1A Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

hypoxia inducible factor 1, alpha subunit

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity).

NP_001300848

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 95-103% - 78-90% - 84-91% - 96-105%
EDTA Plasma (n=5) - 84-93% - 86-96% - 83-99% - 93-101%
Heparin Plasma (n=5) - 92-99% - 82-101% - 93-101% - 80-99%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 93-101 - 96
EDTA Plasma (n=5) - 88-103 - 97
Heparin Plasma (n=5) - 88-95 - 92

Serum, plasma and other biological fluids