HP Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

haptoglobin

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

NP_001119574

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 93-103% - 94-105% - 98-105% - 98-105%
EDTA Plasma (n=5) - 85-104% - 97-105% - 80-102% - 83-95%
Heparin Plasma (n=5) - 94-103% - 88-98% - 89-105% - 92-99%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 89-98 - 93
EDTA Plasma (n=5) - 93-101 - 98
Heparin Plasma (n=5) - 85-92 - 88

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids