IL36G Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

interleukin 36 gamma

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 93-102% - 91-105% - 90-97% - 94-105%
EDTA Plasma (n=5) - 88-95% - 84-95% - 89-96% - 80-103%
Heparin Plasma (n=5) - 84-102% - 82-105% - 83-101% - 78-95%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 92-99 - 95
EDTA Plasma (n=5) - 84-102 - 88
Heparin Plasma (n=5) - 92-99 - 96

Serum, plasma and other biological fluids