IL36G Chemi-Luminescent ELISA Kit (Mouse)

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IL36G

Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (By similarity). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4(+) T-cells and splenocytes.

NP_705731

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 93-101% - 92-99% - 79-92% - 78-94%
EDTA Plasma (n=5) - 81-93% - 86-97% - 88-101% - 98-105%
Heparin Plasma (n=5) - 86-93% - 97-105% - 80-101% - 83-97%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 82-102 - 95
EDTA Plasma (n=5) - 80-101 - 90
Heparin Plasma (n=5) - 81-92 - 86

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids