ADAM10 Chemi-Luminescent ELISA Kit (Rat)

ELISA Kit

Wet Ice

ADAM metallopeptidase domain 10

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.

XP_001054737

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 80-98% - 91-98% - 95-103% - 92-101%
EDTA Plasma (n=5) - 93-102% - 84-92% - 98-105% - 85-104%
Heparin Plasma (n=5) - 86-101% - 98-105% - 86-104% - 81-97%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 99-105 - 102
EDTA Plasma (n=5) - 96-104 - 101
Heparin Plasma (n=5) - 79-93 - 88

Serum, plasma, tissue homogenates and other biological fluids