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LRRC3C Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD03724
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.312 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias leucine-rich repeat-containing protein 3C;putative LRRC3-like protein ENSP00000367157.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
LRRC3C
Gene Fullname
leucine rich repeat containing 3C
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Leucine-rich repeat-containing protein 3C
Nucleotide accession_num
NM_001195545
Protein accession_num
NP_001182474
Protein name
Leucine-rich repeat-containing protein 3C
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
LRRC3C Microplate: 96 Wells (12 x 8 Well strips)
LRRC3C Lyophilized Standard: 2
100X LRRC3C Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Leucine Rich Repeat Containing Protein 3C (LRRC3C) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Leucine Rich Repeat Containing Protein 3C (LRRC3C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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