« Zurück
Startseite /
MMP2 Chemi-Luminescent ELISA Kit (Rabbit)

MMP2 Chemi-Luminescent ELISA Kit (Rabbit)

ArtNr	OKCD03779
Hersteller	AVIVA Systems Biology
Menge	96 Wells
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Typ	Elisa-Kit
Applikationen	CLIA, IA
Specific against	Rabbit (Oryctolagus cuniculus)
Sensitivity	0.078 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	72 kDa gelatinase;72 kDa type IV collagenase;gelatinase A;matrix metallopeptidase 2 (gelatinase A,72kDa gelatinase,72kDa type IV collagenase);matrix metalloproteinase-2;MMP-2.
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	MMP2
Gene Fullname	matrix metallopeptidase 2
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-\|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).
Nucleotide accession_num	NM_001082209
Protein accession_num	NP_001075678
Protein name	72 kDa type IV collagenase
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Mmp2 Microplate: 96 Wells (12 x 8 Well strips) Mmp2 Lyophilized Standard: 2 100X Mmp2 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 Serum (n=5) - 78-92% - 96-104% - 91-98% - 98-105% Heparin Plasma (n=5) - 98-105% - 89-96% - 91-101% - 92-105% - - - -
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 2 (MMP2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 2 (MMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 2 (MMP2) level in the sample or standard.
Kit recovery	Matrix - Recovery range (%) - Average(%) Serum (n=5) - 78-93 - 83 Heparin Plasma (n=5) - 78-97 - 83 - -
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

OKCD03779.pdf

OKCD03779-datasheet.pdf

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 96 Wells

lieferbar

Lieferung vsl. bis 11.12.2025

Express-Lieferung: 7 Tage sparen

Vergleichen

Auf den Wunschzettel

Angebot anfordern