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MSR1 Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD03806
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.312 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CD204;macrophage acetylated LDL receptor I and II;macrophage scavenger receptor type III;macrophage scavenger receptor types I and II;phSR1;phSR2;SCARA1;scavenger receptor class A member 1;SRA;SR-A;SR-AI;SR-AII;SR-AIII.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
MSR1
Gene Fullname
macrophage scavenger receptor 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL) (PubMed:2251254). Isoform III does not internalize acetylated LDL (PubMed:9548586).
Nucleotide accession_num
NM_002445
Protein accession_num
NP_002436
Protein name
Macrophage scavenger receptor types I and II
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
MSR1 Microplate: 96 Wells (12 x 8 Well strips)
MSR1 Lyophilized Standard: 2
100X MSR1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Scavenger Receptor 1 (MSR1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Scavenger Receptor 1 (MSR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Scavenger Receptor 1 (MSR1) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Scavenger Receptor 1 (MSR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Scavenger Receptor 1 (MSR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates, cell lysates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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