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MUC5AC Chemi-Luminescent ELISA Kit (Human)

ArtNr OKCD03811
Hersteller AVIVA Systems Biology
Menge 96 Wells
Kategorie
Typ Elisa-Kit
Applikationen CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.0137 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias gastric mucin;leB;lewis B blood group antigen;major airway glycoprotein;MUC5;mucin;mucin 5,subtypes A and C,tracheobronchial/gastric;mucin 5AC,oligomeric mucus/gel-forming pseudogene;mucin-5 subtype AC,tracheobronchial;mucin-5AC;TBM;tracheobronchial mucin.
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
MUC5AC
Gene Fullname
mucin 5AC, oligomeric mucus/gel-forming
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Gel-forming glycoprotein of gastric and respiratoy tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microrganisms and particles that are subsequently removed by the mucocilary system.
Nucleotide accession_num
NM_001304359
Protein accession_num
NP_001291288
Protein name
Mucin-5AC
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
MUC5AC Microplate: 96 Wells (12 x 8 Well strips)
MUC5AC Lyophilized Standard: 2
100X MUC5AC Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Mucin 5 Subtype AC (MUC5AC) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 Wells
Lieferbar: In stock
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